There is growing interest in automated patch-clamp (APC) assays for ligand-gated targets which are expressed throughout the peripheral and central nervous system. The Acid-Sensing Ion Channel (ASIC) family comprises combinations of ASIC1-4 proteins that form acid-activated cation-selective channels. ASIC channels underlie complex neurological processes and diseases such as cognition, synaptic plasticity, pain, ischemia and epilepsy. ASIC channels are subject to evolutionary predator-prey arms races as shown by potent snake and spider toxins such as Mambalgin-1 and Psalmotoxin-1.