Development and validation of ASIC1a ligand-gated ion channel drug discovery assays on automated patch-clamp platforms (Collaboration with Nanion Technologies)

Poster Description

Acid-sensing ion channels (ASICs) are proton-gated ion channels which are highly sensitive to extracellular acidosis and are permeable to cations1, predominantly Na+. To date, six different ASIC subunits (1a, 1b, 2a, 2b, 3 and 4) encoded by four genes have been identified. Three subunits assemble to form homomeric or heteromeric channels which are expressed throughout the CNS and PNS. Excitatory ASIC channels have a proposed role in nociception and pain, and other neurological diseases such as ischaemia, inflammation and learning and memory.

Download
Recommended Publications
Latest Publications
Using high-throughput automated patch clamp electrophysiology to identify novel TREK-1 modulators in an animal venom library

We developed a high-throughput, electrophysiological assay of TREK-1 function to identify novel modulators. The assay was optimized to identify both activators and inhibitors, providing comprehensive mechanistic data for high value, limited supply screening libraries, such as the venom fraction library used in this study (Targeted Venom Discovery Array, T-VDA, Venomtech, UK).

Scientifica PatchScope Pro: an integrated calcium-imaging and patch-clamp system suitable for selecting specific subsets of neurons for electrophysiology recordings

Scientifica’s PatchScope Pro is an integrated electrophysiology rig incorporating an inverted phase-contrast fluorescence microscope, motorised XY stage and PatchStar micromanipulators, suitable for patch-clamp recording.

View All
Metrion Biosciences

Let’s work together

If you have any questions or would like to discuss your specific assay requirements, we will put you directly in touch with a member of our scientific team. Contact us today to discover more.
Contact us for a quote or discussion
Metrion Biosciences is a contract research organisation (CRO) specialising in high-quality preclinical drug discovery services.
magnifier
linkedin facebook pinterest youtube rss twitter instagram facebook-blank rss-blank linkedin-blank pinterest youtube twitter instagram