We have developed a robust high-throughput automated electrophysiology assay using a monoclonal CHO-hNav1.9 cellular reagent suitable for fully supporting a Nav1.9 discovery program.
Acid-sensing ion channels (ASICs) are proton-gated ion channels which are highly sensitive to extracellular acidosis and are permeable to cations1, predominantly Na+. To date, six different ASIC subunits (1a, 1b, 2a, 2b, 3 and 4) encoded by four genes have been identified. Three subunits assemble to form homomeric or heteromeric channels which are expressed throughout the CNS and PNS. Excitatory ASIC channels have a proposed role in nociception and pain, and other neurological diseases such as ischaemia, inflammation and learning and memory.
We have developed a robust high-throughput automated electrophysiology assay using a monoclonal CHO-hNav1.9 cellular reagent suitable for fully supporting a Nav1.9 discovery program.
Metrion and Sophion present findings that determine whether other insoluble salts can act as seal enhancers and how these solution pairs affect the biophysical properties and pharmacology of the investigated ion channels.