Gigaohm seals (‘gigaseals’) are essential for patch clamp electrophysiology, enabling high-quality recordings by ensuring strong electrical access to cells. In manual patch clamp, seals form between the cell membrane and glass pipette, while in planar patch clamp, they form on a chip substrate. Planar patch clamp often uses seal enhancers like CaF2, which is believed to promote sealing through CaF2 precipitate formation at the solution interface. However, F- can interfere with intracellular components and affect ion channel properties. To address these issues, Sophion developed new seal-enhancing solutions in 2017 (Patent: WO2018100206A1). Metrion and Sophion later collaborated to explore alternative insoluble salts and their impact on ion channels' biophysics and pharmacology.
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We developed a high-throughput, electrophysiological assay of TREK-1 function to identify novel modulators. The assay was optimized to identify both activators and inhibitors, providing comprehensive mechanistic data for high value, limited supply screening libraries, such as the venom fraction library used in this study (Targeted Venom Discovery Array, T-VDA, Venomtech, UK).
We developed a high-throughput, electrophysiological assay of TREK-1 function to identify novel modulators. The assay was optimized to identify both activators and inhibitors, providing comprehensive mechanistic data for high value, limited supply screening libraries, such as the venom fraction library used in this study (Targeted Venom Discovery Array, T-VDA, Venomtech, UK).