We demonstrate the generation and validation of a stable CHO-hHCN2 cell line used as a cellular tool in the successful development of hHCN2 automated electrophysiology screening assays.
Gigaohm seals (‘gigaseals’) are essential for patch clamp electrophysiology, enabling high-quality recordings by ensuring strong electrical access to cells. In manual patch clamp, seals form between the cell membrane and glass pipette, while in planar patch clamp, they form on a chip substrate. Planar patch clamp often uses seal enhancers like CaF2, which is believed to promote sealing through CaF2 precipitate formation at the solution interface. However, F- can interfere with intracellular components and affect ion channel properties. To address these issues, Sophion developed new seal-enhancing solutions in 2017 (Patent: WO2018100206A1). Metrion and Sophion later collaborated to explore alternative insoluble salts and their impact on ion channels' biophysics and pharmacology.
Complete the form below, then remain in this screen and click the download link.
We demonstrate the generation and validation of a stable CHO-hHCN2 cell line used as a cellular tool in the successful development of hHCN2 automated electrophysiology screening assays.
We have developed a robust high-throughput automated electrophysiology assay using a monoclonal CHO-hNav1.9 cellular reagent suitable for fully supporting a Nav1.9 discovery program.