Using automated patch clamp technology, we evaluate the potency and selectivity of ten Nav1.7-selective arachnid peptide toxins, which have been fused to the C-terminus (Fc region) of human IgG1.
Gigaohm seals (‘gigaseals’) are essential for patch clamp electrophysiology, enabling high-quality recordings by ensuring strong electrical access to cells. In manual patch clamp, seals form between the cell membrane and glass pipette, while in planar patch clamp, they form on a chip substrate. Planar patch clamp often uses seal enhancers like CaF2, which is believed to promote sealing through CaF2 precipitate formation at the solution interface. However, F- can interfere with intracellular components and affect ion channel properties. To address these issues, Sophion developed new seal-enhancing solutions in 2017 (Patent: WO2018100206A1). Metrion and Sophion later collaborated to explore alternative insoluble salts and their impact on ion channels' biophysics and pharmacology.
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Using automated patch clamp technology, we evaluate the potency and selectivity of ten Nav1.7-selective arachnid peptide toxins, which have been fused to the C-terminus (Fc region) of human IgG1.
Understanding cardiac safety early is critical in drug development. In their latest poster, Jazz Pharmaceuticals, explain how they utilised Metrion’s clinically translatable cardiotoxicity assay to do exactly that.