Metrion and Sophion present findings that determine whether other insoluble salts can act as seal enhancers and how these solution pairs affect the biophysical properties and pharmacology of the investigated ion channels.
The voltage-gated sodium channels Nav1.7, Nav1.8, and Nav1.9 drive action potentials in nociceptors, with Nav1.9 regulating firing thresholds. There is a significant need for new chronic pain treatments due to the limitations of existing analgesics. While Nav1.7 and Nav1.8 have been widely explored, Nav1.9 is an attractive pain target due to its nociceptor-specific expression and genetic link to pain insensitivity. However, its prosecution has been challenging due to expression difficulties in heterologous systems.
Metrion has developed a monoclonal CHO cell line expressing hNav1.9, validated via manual and automated patch clamp techniques. Using Qube384, we established a high-throughput Nav1.9 assay, recording stable currents with GTPγS and multi-hole acquisition. The sodium channel inhibitor TC-N 1752 showed concentration-dependent inhibition (IC50 0.45 µM). Our robust high-throughput electrophysiology assay provides a reliable platform for Nav1.9 drug discovery.
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Metrion and Sophion present findings that determine whether other insoluble salts can act as seal enhancers and how these solution pairs affect the biophysical properties and pharmacology of the investigated ion channels.
Manual patch-clamp technique was used to evaluate channel pharmacology using cells transiently transfected with wild-type and V434L mutant channel.