Improve efficiency, reduce late-stage failures, and align with regulatory standards by assessing the proarrhythmic liability of your compounds early.
Evaluate the effect of compounds on action potentials recorded from hiPSC-derived cardiomyocytes using conventional manual patch clamp methodology.
Spontaneous or evoked action potentials can be recorded and used to determine the effect of compounds on a range of action potential parameters. The recordings are stable for >30 minutes, which allows the cumulative application of multiple concentrations of each compound.
The manual patch clamp assay generates high fidelity recordings that allow the detection of even subtle changes to the action potential waveform. This helps to successfully discern between compounds with low, medium and high proarrhythmic risk profiles. For example, the figures below show mean data generated using 50 nM dofetilide, which reveals a significant prolongation of all measured APD values.
Figure 1. hiPSC-derived cardiomyocyte action potential screening.
Figure 2. Drug effects on hiPSC-derived cardiomyocyte responses.
Figure 3. Changes in hiPSC-derived cardiomyocyte beat stability.
Improve efficiency, reduce late-stage failures, and align with regulatory standards by assessing the proarrhythmic liability of your compounds early.
"The data is always correct and is in a format that imports directly into our systems, making it so quick and easy for our team to review the data across individual projects."