Validation of an impedance-based phenotypic screening assay able to detect multiple mechanisms of chronic cardiotoxicity in human stem cell-derived cardiomyocytes

Authors

Marc Rogers, CSO Metrion Biosciences

Overview

Presentation by Marc Rogers, CSO Metrion Biosciences, Nanion technologies Exhibitor session, 2020 Biophysical Society meeting, San Diego USA.

Please contact us if you would like any further information regarding our suite of cardiac safety services.

Background

CiPA is designed to address drug-induced cardiac arrythmia (TdP) and the acute effects of drug discovery compounds (minutes – hours) with a focus on plasmalemmal ion channels that underlie the cardiac action potential.

However, new and existing drugs can also cause structural cardiotoxicity, produced by a diverse set of chemical compounds and primary target mechanisms. These chronic effects can appear after days, weeks or months; and not all are reversible.

Classic examples include:

  • Chemotherapy agents used for clinical oncology
  • Anthracyclines such as Doxorubicin (breast cancer)
  • Tyrosine kinase inhibitors (TKI) such as the ‘nibs
  • Proteosome inhibitors such as Bortezomib
  • HDAC inhibitors such as the ‘stats (some of which may also cause QTc prolongation)

Safety pharmacology testing therefore needs a reliable & predictive assay for chronic cardiotoxicity.

Download
Recommended Publications
Latest Publications
M-type K+ channels in nociceptive pathways: physiological roles and therapeutic potential

Presentation from Metrion Biosciences’ external speaker series, Professor Nikita Gamper, University of Leeds, 5th February 2019.

Utility of human iPSC-derived CMs for preclinical safety assays and disease modelling

This talk was presented by Dr Sarah Williams at the Axol Metrion Interactive Stem Cell Forum held in May 2018. This project received funding from the Eurostars-2 joint program with co-funding from the European Union Horizon 2020 Research and Innovation Program.

View All
Metrion Biosciences

Let’s work together

If you have any questions or would like to discuss your specific assay requirements, we will put you directly in touch with a member of our scientific team. Contact us today to discover more.
Contact us for a quote or discussion
Metrion Biosciences is a contract research organisation (CRO) specialising in high-quality preclinical drug discovery services.
magnifier
linkedin facebook pinterest youtube rss twitter instagram facebook-blank rss-blank linkedin-blank pinterest youtube twitter instagram